Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
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چکیده
منابع مشابه
Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI). Using simulations and fixed-cell focal adhesion images, we investigate the complementarity betwee...
متن کاملCombining PALM and SOFI for quantitative imaging of focal adhesions in living cells
Focal adhesions are complicated assemblies of hundreds of proteins that allow cells to sense their extracellular matrix and adhere to it. Although most focal adhesion proteins have been identified, their spatial organization in living cells remains challenging to observe. Photo-activated localization microscopy (PALM) is an interesting technique for this purpose, especially since it allows esti...
متن کاملReview PALM and STORM: Unlocking Live-Cell Super-Resolution
PALM and STORM: Unlocking Live-Cell Super-Resolution Ricardo Henriques, Caron Griffiths, E. Hesper Rego, Musa M. Mhlanga 1 Unidade de Biofisica e Expressão Genetica, Instituto de Medicina Molecular, Faculdade de Medicina Universidade de Lisboa, Lisboa, Portugal 2 Institut Pasteur, Groupe Imagerie et Modelisation, Centre National de la Recherche Scientifique, Unite de Recherche Associe 2582, Par...
متن کاملPALM and STORM: unlocking live-cell super-resolution.
Live-cell fluorescence light microscopy has emerged as an important tool in the study of cellular biology. The development of fluorescent markers in parallel with super-resolution imaging systems has pushed light microscopy into the realm of molecular visualization at the nanometer scale. Resolutions previously only attained with electron microscopes are now within the grasp of light microscope...
متن کاملFast, background-free, 3D super-resolution optical fluctuation imaging (SOFI).
Super-resolution optical microscopy is a rapidly evolving area of fluorescence microscopy with a tremendous potential for impacting many fields of science. Several super-resolution methods have been developed over the last decade, all capable of overcoming the fundamental diffraction limit of light. We present here an approach for obtaining subdiffraction limit optical resolution in all three d...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2016
ISSN: 2041-1723
DOI: 10.1038/ncomms13693